Genetic diagnosis of biomedical materials

Dendritic polymers, known as the "fourth generation of new polymer materials", have attracted wide attention from the scientific community and industry. The novel features of the material are: highly branched molecular structure, a large number of surface functional groups, the presence of cavities within the molecule, the monodisperse molecular weight distribution, and the molecular size of nanometer. The structural characteristics of dendritic molecules make them have unique properties: excellent dissolution and dispersion properties, low melt viscosity and solution viscosity, not easy to crystallize, easy to form films, showing unparalleled nano characteristics.
Dendritic polymers have inherent advantages in the biomedical field due to their high efficiency in gene and drug delivery, solubilization of drug molecules, slow release effect brought by molecular cavities, high permeability and retention effect conferred by nano size, and highly repeatable molecular structure. It can be used in nanomedicine, targeted drug delivery, drug sustained release, gene expression, nuclear magnetic imaging and other fields. At present, dendrimer has achieved good results in gene diagnosis.

Product performance
Advantages of dendrimer in genetic diagnostic applications:
• Fast;
• Efficient;
• Stability;
• Cheap;
• High sensitivity and reproducibility.
Basic physical properties
Take CYD-D850/ poly 2, 6-pyridine dicarboxylic acid membrane DNA electrochemical biosensor for avian virus gene detection as an example:

1. Electrochemical detection shows that the DNA electrochemical probe prepared based on dendrimer has a good ability to select and recognize the target DNA sequences of complete complementarity, one-base mismatch and three-base mismatch, and the DNA fragments of mismatched sequences can be effectively identified by this method. As shown in Figure 1:

(a, b and c are oligonucleotide sequences with complete complementation, one-base mismatch and three-base mismatch, respectively)
2, by measuring the resistance value of the electrode surface to show the precision, reproducibility and stability of the DNA biosensor. As shown in Table 1, the electron transfer resistance values of the DNA biosensor (ssDNA/PAMAM/PDC/Pt electrode and dsDNA /PAMAM/PDC/Pt electrode) before and after hybridization were measured for 7 times in parallel, and the relative standard deviation of Ret and ΔRet difference of the DNA biosensor before and after hybridization was determined. The results were 4.20%, 4.31% and 2.24% respectively, indicating that the electrode based on dendrimer had good precision, stability and reproducibility.
Applicable brands: CYD-D094, CYD-D202, CYD-D418, CYD-D085, etc.